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801 - 809 Article Download PDF View Record in Scopus Google Scholar 2010-03-06 · Background When DNA double-strand breaks (DSB) are induced by ionizing radiation (IR) in cells, histone H2AX is quickly phosphorylated into γ-H2AX (p-S139) around the DSB site. The necessity of DNA-PKcs in regulating the phosphorylation of H2AX in response to DNA damage and cell cycle progression was investigated. Results The level of γH2AX in HeLa cells increased rapidly with a peak level Anti-H2AX pS139 antibody has been tested for use in ELISA and by western blot. Specific conditions for reactivity should be optimized by the end user.
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Cat.No. 10856-1-AP. Phosphorylated H2AX (also termed, gamma-H2AX) functions to recruit and localize DNA repair proteins or cell cycle checkpoint factors to the DNA-damaged sites. In this way, phosphorylated H2AX promotes DNA repair and maintains genomic stability and thus helps prevent oncogenic transformations. Abcam H2ax antibody. select up to five products for comparison. gamma H2A.X (phospho S139) antibody [3F2] Abcam.
I am a PhD scholar working with HL-60 cells and I am trying to induce DNA damage to them and detect gamma H2AX proteins (15kDa) by western blotting using a well-documented toxin, Arsenic.
H2AX phosphorylation in A549 cells induced by the bulky and
Phosphorylated Gamma-H2AX is the first step in the recruitment of the DNA repair protein at the foci site. They are consequently very good biomarkers of DNA double strand break as well as check point for cell cycle arrest following DSB. Measuring gamma-H2AX phosphorylation has become a gold standard in DNA H2AX (pS139) Pure N1-431 100ug. Biosciences Instruments Mouse monoclonal gamma H2A.X (phospho Western blot - Anti-gamma H2A.X (phospho Other Immunogen Type corresponding to Human gamma H2A. SDS extracts were analyzed for H2AX phosphorylation by Western blotting with anti-γ-H2AX antibody.
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1 Product Result | Match Criteria: Product Name, Property Phospho-gamma H2AX pSer139 Antibody Product Specific Information General Information PA1-25001 detects gamma H2A.X (phospho S139) from human and mouse samples. PA1-25001 has been successfully used in Immunoflourescence, and Western blot procedures. By Western Blot a band of ~15 kDa is detected.
However, instead of seeing a band at the expected 15kDA region, I've detected nothing after repeating the experiment several times. The generation of γ-H2AX was also confirmed by western blotting (upper images in Figure 1b and c). In time course experiments, signals of γ-H2AX were detected from 0.5 and 0.25 hours after treatment with 10 −7 and 10 −4 M of 8-MOP plus UVA, respectively (Figure 1d).
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Anti-phospho-Histone H2A.X (Ser139) Antibody, clone JBW301 clone JBW301, Upstate®, from mouse; Synonym: H2AXS139P, Histone H2A.X (phospho S139); find Sigma-Aldrich-05-636 MSDS, related peer-reviewed papers, technical documents, similar products & more at Sigma-Aldrich.
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Samples: Whole cell lysate (15 µg) from Jurkat cells treated with 100 µM etoposide for 4 hours (+) or mock treated (-). Antibody: Affinity purified rabbit anti-gamma-H2AX antibody A300-081A (lot A300-081A-19) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.
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Stain-Free Western Blotting Workflow, Total Protein Normalization, Ordering Information, Resources, Applications & Technologies, Related Products 3 Nov 2017 The overall goal of gamma-H2AX and 53BP1 immunofluorescence microscopy is to analyze the formation and repair of DNA double-strand Western Blot is a workhorse immunoassay for most labs.